| Factor |
Value |
| Molecular
weight (ave.) of DNA base pair |
649
Da |
| Molecular
weight (ave.) of amino acid |
110
Da |
| 1
g/ml DNA |
3.08
µM phosphate |
| 1
g/ml of 1 kb DNA |
3.08
nM 5’ ends |
| 1
mol pBR322 (4363 bp) |
2.83
g |
| 1
pmol linear pBR322 5’ ends |
1.4
g |
| 1
A260 double-stranded DNA |
50
µg/ml |
| 1
A260 single-stranded DNA |
37
µg/ml |
1
kb DNA: 333 amino acids of coding capacity = 36,000 Da
6.5
x 105 Da of double-stranded DNA (sodium salt)
3.3
x 105 Da of single-stranded DNA (sodium salt)
3.4
x 105 Da of single-stranded RNA (sodium salt) |
| 10 kDa protein = 91 amino acids = 273 nucleotides |