|
Factor
|
Value
|
|
Molecular
weight (ave.) of DNA base pair
|
649
Da
|
|
Molecular
weight (ave.) of amino acid
|
110
Da
|
|
1
g/ml DNA
|
3.08
µM phosphate
|
|
1
g/ml of 1 kb DNA
|
3.08
nM 5’ ends
|
|
1
mol pBR322 (4363 bp)
|
2.83
g
|
|
1
pmol linear pBR322 5’ ends
|
1.4
g
|
|
1
A260 double-stranded DNA
|
50
µg/ml
|
|
1
A260 single-stranded DNA
|
37
µg/ml
|
|
1
kb DNA: 333 amino acids of coding capacity = 36,000 Da
6.5
x 105 Da of double-stranded DNA (sodium salt)
3.3
x 105 Da of single-stranded DNA (sodium salt)
3.4
x 105 Da of single-stranded RNA (sodium salt)
|
|
10
kDa protein = 91 amino acids = 273 nucleotides
|
In this Topic
