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Flow Rate Conversion Table

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Factor Value
Molecular weight (ave.) of DNA base pair 649 Da
Molecular weight (ave.) of amino acid 110 Da
1 g/ml DNA 3.08 µM phosphate
1 g/ml of 1 kb DNA 3.08 nM 5’ ends
1 mol pBR322 (4363 bp) 2.83 g
1 pmol linear pBR322 5’ ends 1.4 g
1 A260 double-stranded DNA 50 µg/ml
1 A260 single-stranded DNA 37 µg/ml

1 kb DNA:  333 amino acids of coding capacity = 36,000 Da
6.5 x 105 Da of double-stranded DNA (sodium salt)
3.3 x 105 Da of single-stranded DNA (sodium salt)
3.4 x 105 Da of single-stranded RNA (sodium salt)

10 kDa protein = 91 amino acids = 273 nucleotides
 
 
       
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