Modified RP
Elution properties of MN RP plates in mixtures of
methanol – water and acetonitrile – water
a)
b)
c)
100 80 60 40 20
0 % H O
2
Developing time [min]
for a distance of 7 cm
0
20
40
60
a) RP-18 W,
b) Nano-SIL C18-50,
c) Nano-SIL C18-100
all plates with UV indicator
methanol – water;
acetonitrile – water
Migration of C18-50 and C18-100 silica layers as
compared to RP-18 W plates
Eluent
v/v Migration distances [mm/15 min]
C18-50 C18-100 RP-18 W
Methanol – H O
2
2:1
57
45
44
1:1
52
21
40
1:2
50
0
43
1:3
40
0
45
1:4
30
0
46
0:1
0
0
54
Acetonitrile – H O
2
2:1
62
46
66
1:1
52
30
54
1:2
51
27
46
1:3
48
15
44
1:9
20
0
42
Trichloromethane
68
64
71
For numerous separations with MN RP plates
please visit our on-line application data base at
.
silica layers for HPTLC
Packing Material
RP-18 W/UV254 HPTLC ,Glass plates, Layer thickness: 250 µm
Prod. Code
Price for Unit
`
Plate size [cm]
Pack of [plates]
Preparative TLC, Layer thickness: 1000 µm (1.00 mm)
®
ALUGRAM aluminium sheets , Layer thickness: 150 µm
RP-18 W/UV
254
• Basematerial:
2
• Nano silica 60, specific surface (BET) ~500m /g, mean pore size 60 Å, specific pore volume 0.75mL/g,
particle size 2 - 10 µm
,
For
preparative plates
(1mm thickness of layer) standard silica 60, particle size 5-17 µmpH stability 2 - 10
• Indicator: acid-resistant product with a pale blue fluorescence for short-wave UV (254 nm);
UV-absorbing substances appear as dark-blue to black spots on a light-blue background
• Partial octadecyl (C18) modification, wettable with water, carbon content 14%
• Order of polarity: silica>DIOL>NH >CN>RP-2>C18-50>
RP-18W
>C18-100
2
• NP or RP separation with eluents fromanhydrous solvents tomixtures with high concentrations of water (see table and
figure on previous page) relative polarity of the eluent determines the polarity of the layer.
•
Recommended application:
aminophenol, barbiturates, preservatives, nucleobases, polycyclic aromatic
hydrocarbons, steroids, tetracyclines, Plasticizers (phthalates)
octadecyl-modified HPTLC silica layers
TLC and HPTLC Plates Manufactured by Macherey-Nagel GmbH & Co., Germany
RP-18 W/UV254
811073
10 x 20
50
35990
811075
10 x 10
25
35990
811072
10 x 20
50
75990
811071
20 x 20
25
49990
RP-18 W/UV254
818144
4 x 8
50
15690
818152
5 x 10
50
16750
818145
5 x 20
50
33990
818147
10 x 10
25
17250
818146
20 x 20
25
41990
RP-18 W/UV254
811074
20 X 20
15
76990
Analytical Chromatography
MN
30
