TLC Spray Reagent
Spray reagents allow visualization of the colorless components on a TLC plate. These reagents aremixtures of chemicals
that, when sprayed on the plate and allowed to react, will cause colorless compounds to change to colored compounds
for easy detection.
A spray (visualization) reagent shows selectivity for a specific functional group or compound type. It is an inherent advantage
of TLC/HPTLC that all fractions remain stored on the plate and can be readily derivatized after chromatography. Substances
that do not respond to visible or UV light can be rendered detectable. Inmany cases, substances or classes of substances can
be identified by specific reagents, enabling their selective detection.
Spray reagents to allow visualization of the colorless components on a TLC plate.
TLC Spray Reagent Index
Acids organic
Bromocresol Green
Aldehydes
Fuchsine
Alkaloids
Dragendorff's; Iodoplatinate
Amines
Iodoplatinate; Ehrlich's; Ninhydrin
Amino acids & related compounds
Ninhydrin
Antioxidants
Phosphomolybdic acid
Barbiturates
Mercuric diphenylcarbazone
Flavanoids
Antimony trichloride
Glycopids
Bial's; Orcinol; Diphenylamine
Lipids
Antimony trichloride; Bial's; Orcinol; Bromothymol blue; Cupric acetate; 2,7-
Dichlorofluorescein; Diphenylamine; Dragendorff's; Molybdenum blue;
Ninhydrin; Potassium Dichromate/H2SO4; Rhodamine B; Rhodamine 6G
Phenols
Phosphomolybdic acid; Rhodamine B
Steroids
Antimony pentachloride; Antimony trichloride; Phosphomolybdic acid
Terpenes
Antimony pentachloride
Vitamins
Antimony pentachloride; Iodoplatinate
Charring Reagent
Potassium dichromate/H SO
2
4
Analytical Chromatography
TLC SPRAY REAGENT
PRODUCT CODE
Bromocresol Green Solution
For Detection of Acidic (pKA<5 groups)
02273
2,4-Dinitrophenyl Hydrazine Solution
For Detection Of Aldehyde & Ketones
03474
Iodine Solution
Universal Reagent For Organic Compounds
4200E
Ninhydrin Solution
For Detection of Amino Acids
4912D
Potassium Permanganate Solution
For Detection of Olefins & readily
02273
Oxidized Groups
MN
38
