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RIBOFLAVIN For Biochemistry

Confirms to IP
Vitamin B2

Packings

5 g (00005)

10 g (00010)

25 g (00025)

1GBA000015 1PB1200060 1PB1200100
Article No. 05580 Grade For Biochemistry
Purity 98% CAS No. 83-88-5
Molecular Formula C17H20N4O6 Molecular Weight 376.37
H.S. Code 2936.2310 Shelf Life 60 Months

Physical Properties

Physical state at 20 °C Solid Colour Orange yellow powder
Odour Slight odour pH value 6
Melting point/ Freezing point [°C] 280°C

Specifications

Appearance A yellow to orange yellow crystalline powder Solubility Very slightly soluble in water, more soluble in saline solution than in water;practically insoluble in chloroform.in ethanol (95 per cent) and in ether
Assay (spectrophotometric; on dried basis) 98 - 101% Identification 'A' & 'B' Passes IP tests
pH 5.5 to 7.2 (determined in a saturated solution) Specific optical rotation -135° to 115°(0.5% w/v solution in carbonate free 0.05M NaOH)
Light absorption - at A373 nm/A267 nm 0.31 - 0.33
at A444 nm/A267 nm 0.36 - 0.39 Lumiflavine The filtrate should not more intensely coloured than reference solution BYS6
Related Substances - Impurity A NMT 0.025%
Impurity B NMT 0.2% Impurity C NMT 0.2%
Impurity D NMT 0.2% Any other impurity NMT 0.1%
Total impurities NMT 0.5% Sulphated ash NMT 0.1%
Loss on drying NMT 1.5% (on 1 gm at 105°C)
Revision Date: 12-Oct-2026

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