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Reagents for Molecular Biology
TRIS BUFFER
TRIS BUFFER Molecular Biology
2-Amino-2-(hydroxymethyl)-1,3-propanediol; THAM; Tris base; Tris(hydroxymethyl)aminomethane; Trometamol
Article No.
06391
Grade
Molecular Biology
Purity
99.9%
CAS No.
77-86-1
Molecular Formula
NH
3
C(CH
2
OH)
3
Molecular Weight
121.14
H.S. Code
2922.5090
Shelf Life
60 Months
MSDS
DE
EN
FR
KO
PT
ES
TH
TR
Certificate of Analysis (COAs)
Physical Properties
Physical state at 20 °C
Solid
Colour
White crystalline
pH value
10.5 - 12
Melting point/ Freezing point [°C]
167 - 172 °C
Boiling point [°C]
219 - 220 °C
Initial boiling point [°C]
219°C
Final boiling point [°C]
220 °C
Solubility in water [% weight]
Soluble in water
Packings
100 g
(00100)
500 g
(00500)
Specifications
Appearance
White crystalline powder
Melting point
170 - 173 deg C
pK (at 20°C)
8.3
Solubility (20°C)
800 g/L (H2O)
DNAase/RNAase/Proteases
Not detectable
Assay (titration)
Min 99.9%
pH (1 M in water at 20°C)
10.5 - 11.5
Water (H2O)
Max 0.2%
Copper (Cu)
Max 0.0001%
Iron (Fe)
Max 0.0001%
Lead (Pb)
Max 0.0001%
UV absorbance (1 cm; 1 M in water)
at 260 nm
Max 0.03
at 280 nm
Max 0.02
at 430 nm
Max 0.004
Safety Information and Hazard Symbols
Symbol: GHS07
Signal Word: Warning
Hazardous Statement: H315-H319-H335
Precaution Statement: P261-P305 + P351 + P338
Revision Date :
09-Nov-2013