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TRIS BUFFER Molecular Biology

2-Amino-2-(hydroxymethyl)-1,3-propanediol; THAM; Tris base; Tris(hydroxymethyl)aminomethane; Trometamol

COAs

Packings

100 g (00100)

500 g (00500)

1PW1200100 1PW1200500
Print friendly version
Article No. 06391 Grade Molecular Biology
Purity 99.9% CAS No. 77-86-1
Molecular Formula NH3C(CH2OH)3 Molecular Weight 121.14
H.S. Code 2922.5090 Shelf Life 60 Months

Physical Properties

Physical state at 20 °C Solid Colour White crystalline
pH value 10.5 - 12 Melting point/ Freezing point [°C] 167 - 172 °C
Boiling point [°C] 219 - 220 °C Initial boiling point [°C] 219°C
Final boiling point [°C] 220 °C Solubility in water [% weight] Soluble in water

Specifications

Appearance White crystalline powder Melting point 170 - 173 deg C
pK (at 20°C) 8.3 Solubility (20°C) 800 g/L (H2O)
DNAase/RNAase/Proteases Not detectable Assay (titration) Min 99.9%
pH (1 M in water at 20°C) 10.5 - 11.5 Water Max 0.2%
Copper (Cu) Max 0.0001% Iron (Fe) Max 0.0001%
Lead (Pb) Max 0.0001% UV absorbance -
at 260 nm (1 cm; 1 M in water) Max 0.03 at 280 nm (1 cm; 1 M in water) Max 0.02
at 430 nm (1 cm; 1 M in water) Max 0.004
Revision Date: 21-Feb-2025

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