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Appearance
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A yellow to orange yellow crystalline powder
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Solubility
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Very slightly soluble in water, more soluble in saline solution than in water;practically insoluble in chloroform.in ethanol (95 per cent) and in ether
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Assay (spectrophotometric; on dried basis)
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98 - 101%
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Identification 'A' & 'B'
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Passes IP tests
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pH
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5.5 to 7.2 (determined in a saturated solution)
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Specific optical rotation
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-135° to 115°(0.5% w/v solution in carbonate free 0.05M NaOH)
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Light absorption
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-
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at A373 nm/A267 nm
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0.31 - 0.33
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at A444 nm/A267 nm
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0.36 - 0.39
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Lumiflavine
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The filtrate should not more intensely coloured than reference solution BYS6
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Related Substances
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-
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Impurity A
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NMT 0.025%
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Impurity B
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NMT 0.2%
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Impurity C
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NMT 0.2%
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Impurity D
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NMT 0.2%
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Any other impurity
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NMT 0.1%
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Total impurities
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NMT 0.5%
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Sulphated ash
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NMT 0.1%
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Loss on drying
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NMT 1.5% (on 1 gm at 105°C)
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