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TRIS BUFFER AR

Standard titrimetric substance Specially prepared for biochemistry and enzymology.
2-Amino-2-(hydroxymethyl)-1,3-propanediol; THAM; Tris base; Tris(hydroxymethyl)aminomethane; Trometamol

COAs

Packings

100 g (00100)

500 g (00500)

5000 g (05000)

1PW1200100 1PW1200500 1PW1206500
Print friendly version
Article No. 06392 Grade AR
Purity 99.5% CAS No. 77-86-1
Molecular Formula NH2C(CH2OH)3 Molecular Weight 121.14
H.S. Code 2922.5090 Shelf Life 60 Months

Physical Properties

Physical state at 20 °C Solid Colour White crystalline
pH value 10.5 - 12 Melting point/ Freezing point [°C] 167 - 172 °C
Boiling point [°C] 219 - 220 °C Initial boiling point [°C] 219°C
Final boiling point [°C] 220 °C Solubility in water [% weight] Soluble in water

Specifications

Appearance White crystalline powder Assay (by HClO4 titration; on dried basis) Min 99.5%
pH (5% solution in water) 10 - 11 Melting point 169 - 171°C
Chloride (Cl) Max 0.001% Sulphated ash Max 0.01%
Water Max 0.5% Absorbance (A1%/1cm; 10% in water) -
at 260 nm Max 0.02 at 280 nm Max 0.02
at 300 nm Max 0.02 at 405 nm Max 0.004
at 430 nm Max 0.004 Biological buffer at 25°C pKa 8.07
Revision Date: 21-Feb-2025

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