TRIS BUFFER

TRIS BUFFER AR

Standard titrimetric substance Specially prepared for biochemistry and enzymology.
2-Amino-2-(hydroxymethyl)-1,3-propanediol; THAM; Tris base; Tris(hydroxymethyl)aminomethane; Trometamol

Article No.

06392

Grade

Purity

99.5%

CAS No.

77-86-1

Molecular Formula

NH₂C(CH₂OH)₃

Molecular Weight

121.14

H.S. Code

2922.5090

Shelf Life

60 Months

MSDS

Certificate of Analysis (COAs)

Physical state at 20 °C

Solid

Colour

White crystalline

pH value

10.5 - 12

Melting point/ Freezing point [°C]

167 - 172 °C

Boiling point [°C]

219 - 220 °C

Initial boiling point [°C]

219°C

Final boiling point [°C]

220 °C

Solubility in water [% weight]

Soluble in water

Appearance	White crystalline powder
Assay (by HClO4 titration; on dried basis)	Min 99.5%
pH (5% solution in water)	10 - 11
Melting point	169 - 171°C
Chloride (Cl)	Max 0.001%
Sulphated ash	Max 0.01%
Water (H2O)	Max 0.5%
Absorbance (A1%/1cm; 10% in water)	-
at 260 nm	Max 0.02
at 280 nm	Max 0.02
at 300 nm	Max 0.02
at 405 nm	Max 0.004
at 430 nm	Max 0.004
Biological buffer at 25°C	pKa 8.07